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rna seq
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0
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9
replies
2.6k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 3.6 years ago by
bernatgel
▴ 150 • written 4.6 years ago by
adeler001
• 0
1
vote
5
replies
1.3k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
mahes.muniandy
• 0
2
votes
5
replies
3.2k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.1 years ago by
Steve Lianoglou
★ 13k • written 4.1 years ago by
svlachavas
▴ 830
1
vote
4
replies
902
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 4.5 years ago by
James W. MacDonald
65k • written 4.5 years ago by
abf
▴ 30
3
votes
3
replies
888
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 4.5 years ago by
Leonard Goldstein
▴ 200 • written 4.5 years ago by
116100016
• 0
5
votes
3
replies
973
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 4.9 years ago by
Peter Langfelder
★ 3.0k • written 4.9 years ago by
agustin.gonvi
▴ 20
0
votes
3
replies
653
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.1 years ago by
Kevin Blighe
★ 3.9k • written 4.1 years ago by
isabel.pieterse
• 0
0
votes
2
replies
782
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 4.6 years ago by
Leonard Goldstein
▴ 200 • written 4.6 years ago by
116100016
• 0
1
vote
2
replies
1.9k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.0 years ago
Mozart
▴ 30
0
votes
2
replies
950
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 4.7 years ago by
Michael Love
42k • written 4.7 years ago by
adeler001
• 0
0
votes
1
reply
552
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 4.6 years ago by
Michael Love
42k • written 4.6 years ago by
adeler001
• 0
0
votes
0
replies
497
views
searching for specific RNA sequences
rna seq
4.5 years ago
adeler001
• 0
0
votes
0
replies
1.6k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
6.2 years ago
lawarde.ankita1
▴ 30
13 results • Page
1 of 1
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Replies
Comment: deseq2 results
by
swbarnes2
★ 1.3k
It looks like the PCA plot of a real RNASeq experiment. The red outlier on the left might be the mathematical reason why you have few vali…
Comment: Multi-factorial, longitudinal disease progression analysis with unbalanced patie
by
Dylan.Sheerin
• 0
Thank you very much, Gordon. I'll give voomLmFit a go!
Answer: Multi-factorial, longitudinal disease progression analysis with unbalanced patie
by
Gordon Smyth
50k
Including subjectID in the design matrix always accounts for unbalanced sampling and patient variation but subjects with incomplete records…
Comment: Log-cpm values from limma
by
Gordon Smyth
50k
No, it does not mean that. `voom()` uses the design matrix, including the W covariates, to compute precision weights but not to adjust the …
Comment: Extremely small p-values using Limma for proteomic data
by
Abbey
• 0
This is the code. The only part I changed was adding the contrasts.fit as you suggested. Thanks for taking a look! ``` # Load necessary …
Votes
Answer: Multi-factorial, longitudinal disease progression analysis with unbalanced patie
Answer: Extremely small p-values using Limma for proteomic data
Bioconductor 3.19 is Released!
A: Volcanoplot with limma - RAW P-values or Adj.P-Values
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
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